Roberta Jeane Bezerra Jorge
Federal University of Pernambuco, Brazil
Title: Comparison of renal effects of Bothrops jararaca venoms from southeastern and southern brazilian
Biography
Biography: Roberta Jeane Bezerra Jorge
Abstract
The acute renal failure it is one of main complications in lethal cases of snakebites with Bothrops. A recenty proteomic analysis showed that the B. jararaca venom from southeast (SEv) has more metalloproteinases, while the from south (Sv) has a higher amount of venom PLA2. We have studied the renal effects induced by the Bothops jararaca venom from the two populations from geographic isolated regions within the Brazilian Atlantic rainforest, southeastern(SEv) and a southern(Sv). Isolated kidneys from Wistar rats weighing 250 to 300g (n=6) were perfused with Krebs-Henseleit solution containing 6% of bovine serum albumin previously dialysed for 120 minutes. The effects of the SEv and Sv (10µg/mL) were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP), renal vascular resistance (RVR) and percentage of sodium (%TNa+), potassium (%TK+) and chloride (%TCl-) tubular transport at 60, 90 and 120 minutes of experiment. All data were analyzed by unpaired t test with level of significance of *p<0.05. In the treated group, the addition of the substance occurred 30 minutes (internal control) after the start of the each experiment. Results: The SEv decreased PP and RVR at 60minutes, increased UF at 90 and 120 minutes. Sv decreased GFR at 60, 90 and 120 minutes. It was observed an decrease on the percent of tubular transport of %TNa+, %TK+ and %TCl- at 60, 90 and 120 minutes in the two pools venoms. Discussion and conclusion: Both the venoms, caused significative and different alterations in the renal parameters, and perhaps associated with differences in the protein content of the poison.